Canine CD3 epsilon Protein, His Tag (MALS verified)

Canine CD3 epsilon Protein, His Tag (MALS verified)

• Background: CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
• Description: Canine CD3 epsilon Protein, His Tag (CDE-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Leu 122 (Accession # P27597).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 22 - Leu 122
• Host: HEK 293
• Target: CD3 epsilon
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Canine
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 16.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C