Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

• Background: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Description: Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5 (CD3E) & Q95LI8 (CD3D)).
• Specifications: Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 15kDa, 17kDa, 27-32kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.
• Expression Region: Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)
• Host: HEK 293
• Target: CD3E & CD3D
• Conjugation: Unconjugated
• Tag: C-6×His | C-Flag
• Source: Cynomolgus
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag & Flag Tag (Cat. No. CDD-C52W4) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated BCMA Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.2 ng/mL (Intact assay, QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 15.5 kDa (CD3E) and 14.5 kDa (CD3D)
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C