Beta-2 Microglobulin Antibody

CAT: 0800-R32745Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32745Size:100 µg
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Description
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) . In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, FACS: 1-3ug/10^6 cells
UniProt
P61769
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids Q22-M119 from the human protein were used as the immunogen for the Beta-2 Microglobulin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF/ICC, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Beta-2 Microglobulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Beta-2 Microglobulin antibody is available for research use only.
Storage Conditions
After reconstitution, the Beta-2 Microglobulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with Beta-2 Microglobulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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