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B2M Antibody / Beta 2 Microglobulin

CAT: 0800-RQ4629Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4629Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) . In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL
UniProt
P61769
Host
Mouse
Reactivity
Human, Monkey
Immunogen
Amino acids Q22-M119 from the human protein were used as the immunogen for the B2M antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
2H10
Applications
WB, IHC-P, FACS, IF
Purity
Protein G affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the B2M antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This B2M antibody is available for research use only.
Storage Conditions
After reconstitution, the B2M antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the B2M antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with B2M antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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