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TNF alpha Antibody

CAT: 0800-R30264Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30264Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tumor Necrosis Factor alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It binds and functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation and has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P01375
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 201-233 (QLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL) were used as the immunogen for this TNF alpha antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the TNF alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TNF alpha antibody is available for research use only.
Storage Conditions
After reconstitution, the TNF alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the TNF alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell membrane, secreted
Image Legend
IHC staining of FFPE human B lymphocytic tumor tissue with TNF alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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