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TNF-a Antibody

CAT: 0800-V2900SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2900SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
UniProt
P01375
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 115-130 (NGVELRDNQLVVPSEG) from the human protein were used as the immunogen for the TNF-a antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
P/T2
Applications
FACS, IF, IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the TNF-a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TNF-a antibody is available for research use only.
Storage Conditions
Store the TNF-a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the TNF-a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic and extracellular (secreted)
Image Legend
IHC staining of FFPE human pancreas with TNF-a antibody (clone P/T2) .

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