Human MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH04-24] - BSA and Azide free (Detector)

Human MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH04-24] - BSA and Azide free (Detector)

• Description: MMP-9 (also referred to as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, and type V collagenase) is secreted as a glycosylated proenzyme. Activation of the proenzyme involves proteolytic removal of the N-terminal pro region, resulting in the 82 kDa active enzyme. Active human MMP-9 shares 72% and 74% amino acid sequence identity with mouse and rat MMP-9, respectively. In addition to the zinc-binding site, the catalytic domain also contains three contiguous fibronectin type II homology units responsible for binding gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal hemopexin-like domain. In vitro treatment of the proenzyme with 4-aminophenylmercuric acetate (APMA) produces not only the active enzyme but also a C-terminal truncated form with activity comparable to that of the active form. MMP-9 degrades components of the ECM with high specific activity for denatured collagens (gelatin). It can cleave native collagens of type III, IV, V, and XI, as well as Elastin, Nidogen-1, and Vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. This action can increase or decrease the biological activity of soluble factors and can also liberate them from association with the ECM. MMP-9 can also trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and IL-2 R alpha ).
• Product Name Alternative: 82 kDa matrix metalloproteinase-9 antibody 92 kDa gelatinase antibody 92 kDa type IV collagenase antibody CLG 4B antibody CLG4B antibody Collagenase Type 4 beta antibody Collagenase type IV 92 KD antibody EC 3.4.24.35 antibody Gelatinase 92 KD antibody Gelatinase B antibody Gelatinase beta antibody GelatinaseB antibody GELB antibody Macrophage gelatinase antibody MANDP2 antibody Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody Matrix Metalloproteinase 9 antibody MMP 9 antibody MMP-9 antibody MMP9 antibody MMP9_HUMAN antibody Type V collagenase antibody
• Abbreviation: Matrix metalloproteinase-9 MMP-9 92 kDa gelatinase 92 kDa type IV collagenase Gelatinase B (GELB) 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9 MMP9 CLG4B
• CAS Number: 9000-83-3
• Swiss Prot: P14780 Human
• Cellular Locus: Secreted, extracellular space, extracellular matrix.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant protein within Human MMP9 aa 20-707 (P14780).
• Isotype: IgG
• Clone: PSH04-24
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: ELISA (Det)
• Positive Control: Recombinant Human MMP9 protein (HA210698).
• Concentration: 1 mg/mL
• Dilution: ELISA (Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-23] to Human MMP9 (Capture) (HA722106) and recombinant standard Human MMP9 protein (HA210698). The reference range value is 20.6-5000 pg/ml.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4).
• Molecular Weight: Predicted band size: 78.5 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes