Recombinant Human Interleukin-4 (IL4) (Active)

CAT: 0399-CSB-AP004401HU-01Size: 50 µgDry Ice: NoHazardous: No

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CAT#:0399-CSB-AP004401HU-01Size:50 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4

Abbreviation

Recombinant Human IL4 protein (Active)

Gene Name

IL4

UniProt

P05112

Expression Region

25-153aa

Organism

Homo sapiens (Human)

Target Sequence

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Tag

Tag-Free

Type

Active Protein & In Stock Protein

Source

E.coli

Field of Research

Immunology

Relevance

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 10-70 pg/ml.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Participates in at least several B-cell activation processes as well as of other cell types

Molecular Weight

15.1 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

UniProtKB · P05112

Interleukin-4

IL4_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P05112
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: IL4
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: IL-4
EC number: —
Processing: Precursor
Secondary accessions: Q14630, Q6NZ77

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

B-cell activation

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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Alternative Products

Catalog	Name
MBS2153170-01	Active Interleukin 4 (IL4)
RPU54749-01	Human Interleukin 4 (IL4), Active Protein
GTR19065040-01	Recombinant Human Interleukin-4 (IL4) (Active)