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Recombinant Human Interleukin-4 (IL4) (Active)

CAT: 0013-GTR19065040-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19065040-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Human Interleukin-4 (IL4) spans the amino acid sequence from region 25-153aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Name Alternative
Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
UniProt
P05112
Expression Region
25-153aa
Tag
C-terminal hFc1-MyC-tagged
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Immunology & Inflammation
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
1Measured by its binding ability in a functional ELISA. Immobilized Human IL4 at 2 μg/mL can bind Biotinylated Human IL4R. The EC50 is 7.926-9.836 ng/mL. 2Human IL4 captured on Protein A Chip can bind Recombinant Human IL4R with an affinity constant of 17.1 nM as detected by MetaSPR Assay.
Form
Lyophilized powder
Molecular Weight
43.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

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