Recombinant Mouse CCL2/JE/MCP-1 Protein (Trx Tag)

• Background:

  Monocyte chemoattractant protein 1 (CCL2/JE/MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. CCL2/JE/MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of CCL2/JE/MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.

• Abbreviation:

  CCL2/JE/MCP-1

• Synonyms:

  CCL; Scya; C-C motif chemokine; Small-inducible cytokine A; Monocyte chemotactic protein; Monocyte chemoattractant protein; GDCF; HSMCR;1110006O16Rik;1700006N07Rik; C-C motif chemokine 2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; Small-inducible cytokine A2; SMC-CF; Zcyto; Zcyto7; Je; Mcp1; Scya2; Ccl2; Scya2

• UniProt:

  P10148

• Accession Number:

  P10148

• Expression System:

  E. coli

• Tag:

  N-Trx

• Sequence:

  Gln24-Arg96

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  28 kDa

• Observed Molecular Weight:

  30 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse