JE (MCP-1, CCL2)

• Description:

  The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross reacts across species. Recombinant murine JE is a 13.8 kDa protein containing 125 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

• Synonyms:

  Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594

• NCBI Gene ID:

  20296

• UniProt:

  P10148

• Accession Number:

  NP_035463.1

• Accession Number mRNA:

  NM_011333.3

• Chromosomal Location:

  11 C-E1; 11 46.5 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Human, Mouse, Rabbit, Monkey, Bacteria

• Sequence:

  QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to chemoattract Balb/C mouse spleen MNCs using a concentration range of 1.0-20.0 ng/ml.

• Length:

  125

• Form:

  Lyophilized

• Molecular Weight:

  13.8 kDa

• Host or Source:

  E. coli