Recombinant Mouse MCP-1 Protein (Sumo Tag)

• Background:

  Monocyte chemoattractant protein 1 (CCL2/JE/MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. CCL2/JE/MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of CCL2/JE/MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.

• Abbreviation:

  MCP-1

• Synonyms:

  CCL2; Monocyte chemoattractant protein-1; SCYA2; HC11

• UniProt:

  P10148

• Accession Number:

  P10148

• Expression System:

  E. coli

• Tag:

  N-Sumo

• Sequence:

  Gln24-Arg96

• Field of Research:

  Immunology; Cardiovascular; Cancer

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Calculated Molecular Weight:

  20.9 kDa

• Observed Molecular Weight:

  32 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse