Anti-Hu CD172a PerCP-Cy™5.5

Anti-Hu CD172a PerCP-Cy™5.5

• Background: CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.
• Specifications: The mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Kg-1a cell line
• Target Antigen: CD172a
• Isotype: IgG2a
• Clone: 15-414
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PerCP-Cy1535.5
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Baba T, Badr Mel S, Tomaru U, Ishizu A, Mukaida N: Novel process of intrathymic tumor-immune tolerance through CCR2-mediated recruitment of Sirpα+ dendritic cells: a murine model. PLoS One. 20127(7):e41154. doi: 10.1371/journal.pone.0041154., URL: https://www.ncbi.nlm.nih.gov/pubmed/22815949,*Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J: FcαRI co-stimulation converts human intestinal CD103+ dendritic cells into pro-inflammatory cells through glycolytic reprogramming. Nat Commun. 2018 Feb 289(1):863., URL: https://pubmed.ncbi.nlm.nih.gov/29491406/,
• Other References: *Liu Y, Bühring HJ, Zen K, Burst SL, Schnell FJ, Williams IR, Parkos CA: Signal regulatory protein (SIRPalpha), a cellular ligand for CD47, regulates neutrophil transmigration. J Biol Chem. 2002 Mar 22277(12):10028-36., URL: https://pubmed.ncbi.nlm.nih.gov/11792697/,*Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ: Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood. 2001 May 197(9):2741-9., URL: https://pubmed.ncbi.nlm.nih.gov/11313266/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.