Anti-Hu TCR Cbeta1 PerCP-Cy™5.5

Anti-Hu TCR Cbeta1 PerCP-Cy™5.5

• Background: Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
• Specifications: The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
• Target Antigen: TCR Cbeta1
• Isotype: IgG2a κ
• Clone: JOVI.1
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PerCP-Cy1535.5
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Huppa JB, Ploegh HL: In vitro translation and assembly of a complete T cell receptor-CD3 complex. J Exp Med. 1997 Aug 4186(3):393-403. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9236191,*Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ: Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992 Dec11(6):701-13. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1284120,*San José E, Alarcón B: Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway. J Biol Chem. 1999 Nov 19274(47):33740-6., URL: https://pubmed.ncbi.nlm.nih.gov/10559266/,*Pasare C, Mukherjee P, Verhoef A, Bansal P, Mendiratta SK, George A, Lamb JR, Rath S, Bal V: T cells in mice expressing a transgenic human TCR beta chain get positively selected but cannot be activated in the periphery by signaling through TCR. Int Immunol. 2001 Jan13(1):53-62., URL: https://pubmed.ncbi.nlm.nih.gov/11133834/,*Martin EP, Arnaud J, Alibaud L, Gouaillard C, Llobera R, Huchenq-Champagne A, Rubin B: Molecular mechanisms in the TCR (TCR alpha beta-CD3 delta epsilon, gamma epsilon) interaction with zeta 2 homodimers: clues from a 'phenotypic revertant' clone. Int Immunol. 1999 Jul11(7):1005-15., URL: https://pubmed.ncbi.nlm.nih.gov/10383932/,*Horna P, Shi M, Olteanu H, Johansson U: Emerging role of T-cell receptor constant β chain-1 (TRBC1) expression in the flow cytometric diagnosis of T-cell malignancies. Int J Mol Sci. 2021 Feb 1222(4):1817., URL: https://pubmed.ncbi.nlm.nih.gov/33673033/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.