Anti-Hu CD133 PE-Cy™7

Anti-Hu CD133 PE-Cy™7

• Background: CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
• Specifications: The mouse monoclonal antibody 293C3 recognizes the extracellular epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: human CD133 transfectants
• Target Antigen: CD133
• Isotype: IgG2b
• Clone: 293C3
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PE-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr59(4):391-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/21411810 ,*Angelotti ML, Lazzeri E, Lasagni L, Romagnani P: Only anti-CD133 antibodies recognizing the CD133/1 or the CD133/2 epitopes can identify human renal progenitors. Kidney Int. 2010 Sep78(6):620-1, URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=only+anti-cd133+antibodies+recognizing ,*Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb9(3):216-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=feng+hl+yang+zl+zhang+h ,*Zhang M, Liu Y, Feng H, Bian X, Zhao W, Yang Z, Gu B, Li Z, Liu Y: CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. Am J Pathol. 2013 Feb182(2):565-76. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23195431 ,*Barrantes-Freer A, Renovanz M, Eich M, Braukmann A, Sprang B, Spirin P, Pardo LA, Giese A, Kim EL: CD133 expression is not synonymous to immunoreactivity for AC133 and fluctuates throughout the cell cycle in glioma stem-like cells. PLoS One. 2015 Jun 1810(6):e0130519., URL: https://pubmed.ncbi.nlm.nih.gov/26086074/,*Lardon J, Corbeil D, Huttner WB, Ling Z, Bouwens L: Stem cell marker prominin-1/AC133 is expressed in duct cells of the adult human pancreas. Pancreas. 2008 Jan36(1):e1-6. doi: 10.1097/mpa.0b013e318149f2dc., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=lardon+j+corbeil+d ,*Richardson GD, Robson CN, Lang SH, Neal DE, Maitland NJ, Collins AT: CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci. 2004 Jul 15117(Pt 16):3539-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=richardson+gd+robson+cn,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.