CD74 Antibody

• Description :

  It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-3 µg/mL

• UniProt :

  P04233

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant fragment from the human protein was used as the immunogen for the CD74 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CLIP/7193

• Applications :

  WB, IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD74 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD74 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, paranuclear globular

• Image Legend :

  IHC staining of FFPE human spleen tissue with CD74 antibody (clone CLIP/7193) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.