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COX2 Antibody / MT-CO2

CAT: 0800-RQ7011Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7011Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (called COX2, COII and MT-CO2) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P00403
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids M1-S205) was used as the immunogen for the COX2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the COX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This COX2 antibody is available for research use only.
Storage Conditions
After reconstitution, the COX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the COX2 antibody should be determined by the researcher.
Location
Cytoplasmic (mitochondria)
Image Legend
Western blot testing of 1) rat liver, 2) rat brain, 3) rat small intestine, 4) rat RH35, 5) mouse liver, 6) mouse brain, 7) mouse small intestine and 8) mouse Neuro-2a cell lysate with COX2 antibody. Predicted molecular weight ~25 kDa.

UniProtKB · P00403

Cytochrome c oxidase subunit 2

COX2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00403
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MT-CO2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
7.1.1.9
Processing
—
Secondary accessions
Q37526
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Ligand

CopperMagnesiumMetal-binding

Biological process

Cytochrome c-type biogenesisElectron transportRespiratory chainTransport

Disease

Disease variantPrimary mitochondrial disease

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Molecular function

Translocase

Domain

TransmembraneTransmembrane helix

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