COX2 Antibody / PTGS2

• Description:

  Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (COX2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA) . Cox-1 and COX2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS) . Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. COX2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P35354

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 442-572 was used as the immunogen for the COX2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  COX2/2377

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the COX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This COX2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the COX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the COX2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human prostate cancer with COX2 antibody (clone COX2/2377) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.