MSH2 Antibody

• Description:

  DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms a heterodimer with MSH6 to make the human MutS-alpha mismatch repair complex. It also dimerizes with MSH3 to form the MutS-beta DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

• UniProt:

  P43246

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human MSH2 protein (amino acids Q337-N583) was used as the immunogen for the MSH2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  6B4F7

• Applications:

  WB, IHC-P, IF

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the MSH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This MSH2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the MSH2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the MSH2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.