MSH6 Antibody

• Description:

  The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

• Specifications:

  ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P52701

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein (amino acids 374-540) was used as the immunogen for the MSH6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MSH6/2927

• Applications:

  ELISA, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MSH6 antibody is available for research use only.

• Storage Conditions:

  Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MSH6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human lymph node with MSH6 antibody (clone MSH6/2927) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.