Human Brain-Derived Neurotrophic Factor Recombinant

CAT: 0421-90110-ASize: 2 µgDry Ice: NoHazardous: No

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CAT#:0421-90110-ASize:2 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neuronsin vitro. BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system) .

Description

Recombinant BDNF is a disulfide-linked homodimeric protein consisting of two 119 amino acid residues. migrates as an approximately 27 kDa protein under non-reducing conditions and as a 13-14 kDa protein under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Brain Derived Neurotrophic Factor mature chain was expressed inE. coli.

UniProt

P23560

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 97% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

14 kDa

Formulation

Lyophilized from 0.2 µm filtered citric acid, pH 5.7.

UniProtKB · P23560

Neurotrophic factor BDNF precursor form

BDNF_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P23560
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: BDNF
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: proBDNF
EC number: —
Processing: Precursor
Secondary accessions: A7LA85, A7LA92, D3DQZ2, Q598Q1, Q6DN19, Q6YNR2, Q6YNR3, Q9BYY7, Q9UC24

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative promoter usageAlternative splicing

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Growth factor

Cellular component

Secreted

Domain

Signal

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Alternative Products

Catalog	Name
545494	Brain Derived Neurotrophic Factor
AP80860	Brain-derived neurotrophic factor
303151	BDNF (Abrineurin, Brain Derived Neurotrophic Factor, Brain-derived Neurotrophic Factor, Neurotrophin)
22060262-1	Human Brain-Derived Neurotrophic Factor (Recombinant)
45105P-1000	Recombinant Human Brain-Derived Neurotrophic Factor
MBS2030737-01	Recombinant Brain Derived Neurotrophic Factor (BDNF)