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POP Assay Kit

CAT: 0421-80106Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-80106Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Prolyl oligopeptidase (POP), also known as prolyl endopeptidase (PREP), is a cytosolic serine peptidase involved in the maturation and degradation of peptide hormones and neuropeptides.
Description
The Fluorogenic Prolyl OligoPeptidase (POP) Assay Kit is a complete assay system designed to measure activity of the purified POP enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive materials and chromatography in traditional assays. Purified human recombinant POP is included in the kit as a positive control. Using this kit, only one simple step, in which the fluorometric substrate is incubated with purified POP, is needed to analyze the POP activity level. The resulting fluorescent product can then be easily measured with a microtiter-plate fluorimeter.Need us to run inhibitor screens or profile your compounds against POP? Check out ourProtease Screening Services.This product has beencited 5 times.
UniProt
P48147
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Conditions
-80°C
Storage Conditions
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Notes
The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt

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