PDE1C Assay Kit

• Background:

  Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium.

• Description:

  The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent is added  to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate readercapable of measuring fluorescence polarization.

• UniProt:

  Q14123

• Applications:

  Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

• Format:

  COMPONENTS:

• Shipping Conditions:

  -80°C (Dry Ice)

• Storage Conditions:

  At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes:

  MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:Fluorescent microplate reader capable to measure fluorescence polarization