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TYR Phosphorylation Assay Kit

CAT: 0421-79502Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-79502Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TYR (tyrosine) phosphorylation is a modification of proteins where a phosphate group is added to the amino acid tyrosine by tyrosine kinases. This modification is crucial to regulate enzyme activity and signaling pathways, such as the Ras-MAPK (mitogen activated protein kinase) signaling pathway, and is needed for proteins involved in cell differentiation, cell cycle, gene regulation and transcription, angiogenesis and many other functions. Tyrosine kinases can be receptor or non-receptor, with RTK (receptor tyrosine kinases) being transmembrane proteins that can bind ligands extracellularly, while NRTK are mostly soluble intracellular proteins. Tyrosine phosphorylation can be reverted by PTPs (tyrosine phosphatases) . Abnormal tyrosine phosphorylation is linked to several diseases, such as cancer and diabetes. An understanding of the TYR phosphorylation state of a protein and how to manipulate it, via the partners involved, is critical for the development of targeted therapies for tyrosine phosphorylation linked diseases.
Description
The TYR Phosphorylation Assay Kit is designed for measuring TYR (tyrosine) phosphorylation and screening and profiling small molecules that affect it. This kit comes in a convenient 96-well format, with enough antibody specific for phosphorylated TYR residues, HRP-labeled antibody and assay buffer for 100 reactions. This kit also includes phosphorylated JAK2 (Janus kinase 2) as standard control.
UniProt
P51160
Applications
Detect TYR phosphorylation in recombinant proteins.Screening and profiling of small molecules that can alter TYR phosphorylation
Format
Catalog #NameAmountStorage52140Z5Primary Antibody 3012.5 µl-80°C52130HSecondary HRP-Labeled Antibody 110 µl-80°C1x Phosphorylation Buffer3 x 1 ml-20°C79956Blocking Buffer50 ml+4°CPhosphorylated JAK2*50 µl-80°CHRP Chemiluminescence Substrate A (translucent bottle) 6 ml+4°CHRP Chemiluminescence Substrate B (brown bottle) 6 ml+4°C96-well strip plate (coated) 1 plateRoomTemp* The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.

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