CD38 (Mouse) Fluorogenic Assay Kit (Cyclase Activity)

• Background :

  CD38, a differentiation antigen of B lymphocytes, is a type II integral membrane protein. It is also known as ADP-ribosyl cyclase and nicotinamide adenine dinucleotide (NAD+) glycohydrolase. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in various cells, including pancreatic b cells, where it regulates insulin secretion. CD38 is a prognostic biomarker for acute B lymphoblastic leukemia, and anti-CD38 antibodies are in clinical trials as therapeutics for multiple myeloma.

• Description :

  The CD38 (Mouse) Inhibitor Screening Assay Kit (Cyclase Activity) is designed to measure the cyclase activity of CD38 (mouse) for screening and profiling applications. The CD38 assay kit comes in a convenient 96-well format, with purified recombinant CD38 enzyme, its substrate nicotinamide guanine dinucleotide (NGD+), and CD38 assay buffer for 100 enzyme reactions. In addition, the kit includes the CD38 inhibitor quercetin for use as an inhibitor control.Assay PrincipleNeed us to run inhibitor screens or profile your compounds against CD38 (Mouse) (Cyclase Activity) ? Check out ourImmunotherapy Biochemical Screening Services.

• Applications :

  Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

• Format :

  Catalog #NameAmountStorage79070CD38, His-Tag (Mouse), HiP™2 x 1 µg-80°C3x CD38 cyclase assay buffer4 ml-20°CCD38 substrate NGD+50 µl-20°CQuercetin (50 mM in DMSO) 100 µl-20°C79685Black 96-well plate1Room Temp.*The initial concentration of CD38 is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions :

  -80°C

• Storage Conditions :

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes :

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications :

  The final concentration of DMSO in the reaction should be ≤1%.