PD-1:PD-L2 Homogeneous Assay Kit

• Description:

  The PD-1:PD-L2 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L2 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.Need us to run inhibitor screens or profile your compounds against PD-1:PD-L2? Check out ourImmunotherapy Biochemical Screening Services.

• UniProt:

  PD-1: Q15116; PD-L2: Q9BQ51

• Applications:

  Useful for screening for inhibitors of PD-1 binding to PD-L2

• Format:

  COMPONENTS:

• Shipping Conditions:

  -80°C (Dry Ice)

• Storage Conditions:

  Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes:

  AlphaScreen®and AlphaLISA®are registered trademarks of PerkinElmer, Inc.

• Contraindications:

  Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1:PD-L2 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+) . The presence of > 1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.