PD-L2 Antibody

• Description:

  Recognizes a protein identified as PD-L2 (same as PDCD1LG2) . Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator) . The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

• UniProt:

  Q9BQ51

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PDL2/2676

• Applications:

  ELISA, FACS, IF, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the PD-L2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PD-L2 antibody is available for research use only.

• Storage Conditions:

  Store the PD-L2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the PD-L2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, cell surface

• Image Legend:

  Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone PDL2/2676) and Reddot nuclear stain (red) .