Products for Research Use Only
RegisterLogin

PDE1C (Mouse) Assay Kit

CAT: 0421-61312Size: 96 ReactionsDry Ice: YesHazardous: No
Product image 1
1 / 1
CAT#:0421-61312Size:96 Reactions
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Mouse PDE1C, also known as cAMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility.Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates.  Beads selectively bind the phosphate group in the nucleotide product.  This increases the size of the nucleotide relative to unreacted cyclic monophosphate.  In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited.  Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization.  Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.
Description
The Mouse PDE1C Assay Kit is designed for identification of inhibitors of Mouse PDE1C using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE1C to the binding agent.The Mouse PDE1C Assay Kit comes in a convenient 96-well format, with purified Mouse PDE1C enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to theMouse PDE1C Assay Kitis the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.Need us to run inhibitor screens or profile your compounds against PDE1C (Mouse) ? Check out ourPhosphodiesterase Screening Services.
UniProt
Q64338
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Format
Components
Shipping Conditions
-80°C
Storage Conditions
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Notes
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:Fluorescent microplate reader capable to measure fluorescence polarization
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt

Related Products

CatalogName
60312PDE1C Assay Kit
60382PDE1C Assay Kit
60705PDE1C TR-FRET Assay Kit
79663PDE1B (Mouse) Assay Kit
60384-1PDE4C Assay Kit
60311PDE1B Assay Kit
60384-2PDE4C Assay Kit
79501PDE6C Assay Kit
60310PDE1A Assay Kit
79501-2PDE6C Assay Kit

Alternative Products

CatalogName
GWB-PS7A7FPDE1C Assay Kit
137330PDE1C Assay Kit, BioAssayTM
CRM2993-01PDE1C siRNA (Mouse)
36279055PDE1C Adenovirus (Mouse)
abx928068-01Mouse PDE1C siRNA
RP160895PDE1C Recombinant Protein (Mouse)
E046654Mouse PDE1C ELISA Kit
abx971967-01Mouse PDE1C shRNA Plasmid
OKEH03612-96WPDE1C ELISA Kit (Mouse)
171962PDE1C Mouse BioAssayTM Kit

Popular Products