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PDE1C ELISA Kit (Mouse)

CAT: 0247-OKEH03612-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03612-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Phosphodiesterase 1C
Gene Aliases
3',5'-cyclic-AMP phosphodiesterase;3',5'-cyclic-GMP phosphodiesterase; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; cam-PDE 1C.
Gene ID
18575
Accession Number
NP_001020739.1
Reactivity
Mouse, Mus musculus
Target
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDE1C ELISA Kit (Mouse) (OKEH03612) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pde1c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pde1c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pde1c captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
80 kDa
Specificity
Natural and recombinant Mouse Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Protein Length
706
NCBI Gene Symbol
Pde1c
Protein Name
Q64338
Gene Name URL
Pde1c
Nucleotide Accession Number
NM_001025568.2

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