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HDAC2 Kinetic Assay Kit

CAT: 0421-53002Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-53002Size:96 Reactions
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Description
The Kinetic HDAC2 Assay Kit is a complete assay system designed to measure histone deacetylase 2 (HDAC2) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC2 reaction on a microtiter plate is needed and then the HDAC2 activity level can be measured kinetically using a fluorescence reader.This product has beencited 5 times.
UniProt
Q92769
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors of HDAC2 for drug discovery and HTS applications.
Shipping Conditions
-80°C
Storage Conditions
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt

UniProtKB · Q92769

Histone deacetylase 2

HDAC2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92769
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HDAC2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HD2
EC number
3.5.1.98
Processing
—
Secondary accessions
B3KRS5, B4DL58, E1P561, Q5SRI8, Q5SZ86, Q8NEH4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Biological rhythmsTranscriptionTranscription regulation

Molecular function

Chromatin regulatorHydrolaseRepressor

Cellular component

CytoplasmNucleus

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