HDAC6 Fluorogenic Assay Kit

• Background:

  HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.

• Description:

  The Fluorogenic HDAC6 Assay Kit is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. In addition, the kit includes purified HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against HDAC6? Check out ourDeacetylase/Sirtuin Screening Services.This product has beencited 25 times.

• UniProt:

  Q9UBN7

• Applications:

  Useful for studying enzyme kinetics and screening small molecular inhibitors of HDAC6 for drug discovery and HTS applications.

• Format:

  96 ReactionsCatalog#ComponentAmountStorage50006HDAC6 human recombinant enzyme9 µg-80°CAvoidfreeze/thawcycles!50037Fluorogenic HDAC substrate 3 (5 mM) 25 µl-80°C500302x HDAC Developer (contains Trichostatin A) (2 µM) 6 ml-80°CTrichostatin A in DMSO (200 µM) 100 µl-20°C50031HDAC Assay Buffer10 ml-20°C79685Black, low binding NUNC black microtiter plate1Room Temp384 ReactionsCatalog #ComponentAmountStorage50006HDAC6 human recombinant enzyme9 µg-80°CAvoidfreeze/thawcycles!50037Fluorogenic HDAC substrate 3 (5 mM) 50 µl-80°C500302x HDAC Developer (contains Trichostatin A) (2 µM) 2 x 6 ml-80°C50031HDAC Assay Buffer10 ml-20°C79961384-well black, low binding microtiter plate1Room Temp

• Shipping Conditions:

  -80°C

• Storage Conditions:

  6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Contraindications:

  DMSO > 1%, strong acids or bases, ionic detergents, high salt