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HDAC1 Kinetic Assay Kit

CAT: 0421-53001Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-53001Size:96 Reactions
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Description
The Kinetic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC1 reaction on a microtiter plate is needed and then the HDAC1 activity level can be measured kinetically using a fluorescence reader.This product has beencited 10 times.
UniProt
Q13547
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors of HDAC1 for drug discovery and HTS applications.
Format
Catalog #ReagentAmountStorage50051HDAC1 human recombinant enzyme10 µg-80°CAvoid Freeze/Thaw cycles50037Fluorogenic HDAC substrate 3 (5 mM) 50 µl-80°C5302910x HDAC Developer1 ml-80°CDeveloper Dilution Buffer10 ml-20°CSAHA (1 mM) 20 µl-20°C50031HDAC Assay Buffer20 ml-20°CBSA (1mg/ml) 1 ml-20°C79685black, low binding NUNC blackmicrotiter plate1Room Temp
Shipping Conditions
-80°C
Storage Conditions
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt

UniProtKB · Q13547

Histone deacetylase 1

HDAC1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13547
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HDAC1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HD1
EC number
3.5.1.98
Processing
—
Secondary accessions
Q92534
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinS-nitrosylationUbl conjugation

Biological process

Biological rhythmsTranscriptionTranscription regulation

Molecular function

Chromatin regulatorHydrolaseRepressor

Ligand

Metal-bindingZinc

Cellular component

Nucleus

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