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PRMT4 Homogeneous Assay Kit

CAT: 0421-52068Size: 384 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-52068Size:384 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PRMT4 Homogeneous Assay Kit is designed to measure PRMT4 activity for screening and profiling applications. PRMT4 (CARM1) is an arginine methyltransferase and dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3. The PRMT4 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT4 substrate, primary antibody, methylation assay buffer, and purified PRMT4 for 384 enzyme reactions. The key to the PRMT4 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT4 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Need us to run inhibitor screens or profile your compounds against PRMT4? Check out ourMethyltransferase Screening Services.
UniProt
Q86X55
Applications
Useful for studying enzyme kinetics and HTS applications.
Format
Catalog #ComponentAmountStorage51047PRMT4/CARM1*60 µg-80°CAvoidfreeze/thawcycles!52120100 μM S-adenosylmethionine250 µl-80°C52140Z3Primary antibody 2820 µl-80°CPRMT4 substrate300 µl-80°C4x PRMT4 assay buffer (add 30 µl of 500 mM DTT before experiment) 3 ml-20°C4x Detection buffer2 ml-20°C*The concentration of PRMT4 is lot-specific and will be indicated on the tube containing the enzyme
Shipping Conditions
-80°C
Storage Conditions
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Notes
AlphaScreen®and AlphaLISA®are registered trademarks of PerkinElmer, Inc.
Contraindications
Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+) . The presence of > 1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

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