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Phospho-Histone H3 Antibody (pS10)

CAT: 0800-V9213-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9213-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%) . Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002) . For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P68431
Host
Mouse
Reactivity
Human
Immunogen
Amino acids ARK (pS) TGGKAPRKQLc) were used as the immunogen for the phospho-Histone H3 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RPHH3/6824
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the phospho-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Histone H3 antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the phospho-Histone H3 antibody should be determined by the researcher.
Location
Nucleus, Chromosome
Image Legend
Western blot testing of human MCF7 cell lysate with phospho-Histone H3 antibody.

UniProtKB · P68431

Histone H3.1

H31_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P68431
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
H3C1, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3C10, H3C11, H3C12
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A0PJT7, A5PLR1, P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9, Q6NXU4, Q71DJ3, Q93081
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationCitrullinationHydroxylationIsopeptide bondLipoproteinMethylationPhosphoproteinUbl conjugation

Cellular component

ChromosomeNucleosome coreNucleus

Disease

Disease variant

Molecular function

DNA-binding
Product Datasheet
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