Phosphorylated Histone H3 Antibody / pS10

• Description:

  Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%) . Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002) . For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P68431

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids ARK-pS-TGGKAPRKQLc of Phosphohistone H3 (phospho S10) were used as the immunogen for the Phosphorylated Histone H3 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  RHH3/9924

• Applications:

  IHC-P, WB

• Format:

  Purified

• Limitations:

  This Phosphorylated Histone H3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Phosphorylated Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Phosphorylated Histone H3 antibody should be determined by the researcher.

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human tonsil tissue with Phosphorylated Histone H3 antibody (clone rHH3/9924) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.