E2F6 Antibody

• Description:

  The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  O75461

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human E2F6 protein was used as the immunogen for the E2F6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  PCRP-E2F6-1F8

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the E2F6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This E2F6 antibody is available for research use only.

• Storage Conditions:

  Aliquot the E2F6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the E2F6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  Immunofluorescent staining of human HeLa cells using E2F6 antibody (green, clone PCRP-E2F6-1F8) and phalloidin (red) .