E2F4 Antibody

CAT: 0800-V4414-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V4414-100UGSize:100 µg
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Description
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Q16254
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the E2F4 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
E2F4/8811R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the E2F4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This E2F4 antibody is available for research use only.
Storage Conditions
Aliquot the E2F4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the E2F4 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
IHC staining of FFPE human colon cancer tissue with E2F4 antibody (clone E2F4/8811R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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