Recombinant CD8A Antibody

• Description :

  CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) . A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P01732

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 135-235 of CD8 alpha protein was used as the immunogen for the recombinant CD8A antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG1 k

• Clone :

  RCD8/6590

• Type :

  Recombinant

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Aliquot the recombinant CD8A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This recombinant CD8A antibody is available for research use only.

• Storage Conditions :

  Aliquot the recombinant CD8A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Cell Surface

• Image Legend :

  IHC staining of FFPE human lymph node with recombinant CD8A antibody (clone rCD8/6590) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.