Recombinant Bcl-2 Antibody

• Description:

  This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P10415

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RBCL2/6418

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant Bcl-2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm, nuclear membrane

• Image Legend:

  Western blot testing of human HDLM-2 cell lysate with recombinant Bcl-2 antibody. Predicted molecular weight ~26 kDa.