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Recombinant Bcl-2 Antibody

CAT: 0800-V8937SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8937SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P10415
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RBCL2/6418
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Bcl-2 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.
Location
Cytoplasm, nuclear membrane
Image Legend
Western blot testing of human HDLM-2 cell lysate with recombinant Bcl-2 antibody. Predicted molecular weight ~26 kDa.

UniProtKB · P10415

Apoptosis regulator Bcl-2

BCL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10415
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BCL2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
C9JHD5, P10416, Q13842, Q16197
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Biological process

ApoptosisAutophagy

Cellular component

CytoplasmEndoplasmic reticulumMembraneMitochondrionMitochondrion outer membraneNucleus

Disease

Disease variantProto-oncogene

PTM

PhosphoproteinUbl conjugation

Domain

TransmembraneTransmembrane helix

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