Recombinant Bcl-2 Monoclonal Antibody

• Background:

  Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability. Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

• Abbreviation:

  BCL2

• UniProt:

  P10415

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide corresponding to the N-terminus of the Human Bcl-2.

• Target:

  B-cell Lymphoma; PPP1R; BCL; Bcl-2; PPP1R50; BCL2; apoptosis regulator Bcl-2; B-cell Lymphoma 2; apoptosis regulator Bcl-2; Bcl-2; PPP1R50; Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B-cell; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma; Oncogene B-cell leukemia 2; Protein phosphatase 1; regulatory subunit 50; Bcl2

• Clonality:

  Monoclonal

• Clone:

  8D4

• Conjugation:

  Unconjugated

• Applications:

  IHC-P; FCM

• Field of Research:

  Cell Biology; Signal Transduction; Cancer; Kits; Lysates; Other; Metabolism

• Purification:

  Protein A

• Concentration:

  1 mg/mL

• Dilution:

  IHC-P 1:250-1:1000; FCM 1:25-1:100

• Buffer:

  0.2 μm filtered solution in PBS

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

• Isotype:

  IgG