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Recombinant Bcl-2 Antibody

CAT: 0800-V3543SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3543SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P10415
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RBCL2/796
Type
Recombinant
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Bcl-2 antibody is available for research use only.
Storage Conditions
Store the recombinant Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic, membrane

UniProtKB · P10415

Apoptosis regulator Bcl-2

BCL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10415
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BCL2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
C9JHD5, P10416, Q13842, Q16197
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Biological process

ApoptosisAutophagy

Cellular component

CytoplasmEndoplasmic reticulumMembraneMitochondrionMitochondrion outer membraneNucleus

Disease

Disease variantProto-oncogene

PTM

PhosphoproteinUbl conjugation

Domain

TransmembraneTransmembrane helix

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