CD36 Antibody

• Description:

  Recognizes a protein of 80kDa-90kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN) . CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P16671

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human CD36 protein was used as the immunogen for the CD36 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  CD36/7217

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD36 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD36 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD36 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD36 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC staining of FFPE human spleen tissue with CD36 antibody (clone CD36/7217) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.