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Recombinant Napsin A Antibody

CAT: 0800-V8740-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8740-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
O96009
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 50-150 from the human protein was used as the immunogen for the recombinant Napsin A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
NAPSA/4400R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Napsin A antibody is available for research use only.
Storage Conditions
Store the recombinant Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Napsin A antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human lung adenocarcinoma with recombinant Napsin A antibody (clone NAPSA/4400R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · O96009

Napsin-A

NAPSA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O96009
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NAPSA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.23.-
Processing
Precursor
Secondary accessions
Q8WWD9
Protein keywords

Molecular function

Aspartyl proteaseHydrolaseProtease

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Cellular component

Secreted

Domain

Signal

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