Napsin A Antibody

• Description:

  Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  O96009

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment corresponding to amino acids 189-299 from the human protein was used as the immunogen for the Napsin A antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  NAPSA/1238

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Napsin A antibody is available for research use only.

• Storage Conditions:

  Store the Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human lung Adenocarcinoma stained with Napsin-A antibody (clone NAPSA/1238) .