Myelin Basic Protein Antibody

• Description:

  Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4275 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. MBP/4275 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P02686

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Myelin Basic Protein was used as the immunogen for this Myelin Basic Protein antibody.)

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  MBP/4275

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Myelin Basic Protein antibody is available for research use only.

• Storage Conditions:

  Store the Myelin Basic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4275) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.