OX40 Antibody

• Description:

  This MAb recognizes a protein of 43kDa, identified as OX40, which is also known as CD134. OX40 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. It is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. It functions as a T cell co-stimulatory receptor when bound by OX40 Ligand / TNFSF4 that is expressed by antigen presenting cells. OX40 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. OX40 is upregulated at the sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.

• Specifications:

  ELISA: order Ab without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P43489

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 59-205 from the human protein was used as the immunogen for the OX40 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  OX40/3427

• Applications:

  ELISA, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the OX40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This OX40 antibody is available for research use only.

• Storage Conditions:

  Store the OX40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the OX40 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membranous

• Image Legend:

  IHC staining of FFPE human tonsil with OX40 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.