Recombinant CD8A Antibody

CAT: 0800-V8519-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V8519-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide.CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8 occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P01732
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 135-235 from the human protein was used as the immunogen for the recombinant CD8A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
CD8/4391R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD8A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD8A antibody is available for research use only.
Storage Conditions
Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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