Recombinant CD8A Antibody

• Description:

  The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide.CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8 occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P01732

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 135-235 from the human protein was used as the immunogen for the recombinant CD8A antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  CD8/4391R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the recombinant CD8A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant CD8A antibody is available for research use only.

• Storage Conditions:

  Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface

• Image Legend:

  IHC staining of FFPE human lymph node tissue with recombinant CD8A antibody (clone CD8/4391R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.