CD20 Antibody

• Description :

  Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.12. Workshop IV; Code B17) . It recognizes an extracellular domain of CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.

• CAS Number :

  9007-83-4

• Specifications :

  Functional Studies: order Ab without BSA, ELISA: order Ab without BSA for coating

• UniProt :

  P11836

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Stimulated human leukocytes were used as the immunogen for the CD20 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG3 κ

• Clone :

  109-3C2

• Applications :

  Functional Studies, ELISA

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD20 antibody is available for research use only.

• Storage Conditions :

  Store the CD20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD20 antibody should be determined by the researcher.

• Location :

  Predominantly cell surface with some cytoplasmic